Ligand substitution reactions of metallothioneins with EDTA and apo-carbonic anhydrase.

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Ligand substitution reactions of metallothioneins with EDTA and apo-carbonic anhydrase.

The reactions of Zn-, Zn,Cd-, and Cd-thioneins with EDTA and apo-carbonic anhydrase have been studied. The ligand substitution reaction of zinc with EDTA is multiphasic, having both associative and dissociative components in the rate expression. The cadmium sites are about 2 orders of magnitude less reactive. In contrast, apo-carbonic anhydrase abstracts zinc from Zn-thionein and Zn,Cd-thionein...

متن کامل

Interaction of native and apo-carbonic anhydrase with hydrophobic adsorbents: A comparative structure-function study.

Our previous studies indicated that native carbonic anhydrase does not interact with hydrophobic adsorbents and that it acquires this ability upon denaturation. In the present study, an apo form of the enzyme was prepared by removal of zinc and a comparative study was performed on some characteristic features of the apo and native forms by far- and near-UV circular dichroism (CD), intrinsic flu...

متن کامل

Ligand-Induced Protein Mobility in Complexes of Carbonic Anhydrase II and Benzenesulfonamides with Oligoglycine Chains

This paper describes a biophysical investigation of residual mobility in complexes of bovine carbonic anhydrase II (BCA) and para-substituted benzenesulfonamide ligands with chains of 1-5 glycine subunits, and explains the previously observed increase in entropy of binding with chain length. The reported results represent the first experimental demonstration that BCA is not the rigid, static gl...

متن کامل

Investigation of solvent effect on the active site energy of Carbonic Anhydrase and Ribonucleotide Reductase

Enzymes catalyze many biological reactions. The rates of chemical reaction in the presence ofenzymes are, in some cases, accelerated more than 10 orders of magnitude relative to thecorresponding rates in solution.In this paper a comparison between optimized structures of two enzyme molecules in aspect ofenergy and dipole moment in different conditions including presence of metallic ion, without...

متن کامل

Carbonic anhydrase under pressure

Investigations of the rapid enzyme carbonic anhydrase have now been extended by crystallographic analysis at high CO2 pressures to examine the movements of water molecules in different steps of the catalysis. The rate of catalysis seems well explained by the assembled observations.

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1980

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.77.11.6334